WORKINGGROUP 2
  • Participants:

    Participant
    Organization
    E-mail
    Phone number
    Fax
    Dr. Axel Cloeckhart  INRA, France  axel.cloeckaert@tours.inra.fr  /  / 
    Dr. Bernd Appel  /  b.appel@bfr.bund.de  /  / 
    Dr. Constantinos Sakarellos  Univeristy of Ioannina, Ioannina, Greece  ksakarel@cc.uoi.gr  /  / 
    Dr. Louis Moreno  UCL, Brussels, Belgium  moreno@dice.ucl.ac.be  /  / 
    Dr. Maria Nica  Dr Victor Babes Hospital for Infectious/Tropical Diseases and Dr. Victor Babes Foundation, Bucharest  centru@cdt-babes.ro / iuliana_a@yahoo.com  0040 21 31 79 503  0040 21 31 79 502 
    Dr. Maria Sakarellos-Daitsiotis  The Laboratory of Peptide Chemistry, Department of Chemistry, University of Ioannina, Ioannina, Gree  msakarel@cc.uoi.gr  0030 65 19 83 90  0030 65 14 58 40 
    Dr. Natascha Helena Beyer / Dr. Niels Heegaard  The Statens Serum Institute, Copenhagen, Denmark  nat@ssi.dk  0045 32 68 32 68  0045 32 68 38 68 
    Dr. Patricia Renesto  The Unite des Rickettsies CNRS UMR 6020 - IFR 48 - Faculte de Medecine, Marseille, France  patricia.renesto@medecine.univ-mrs.fr  0033 49 13 24 625  0033 49 13 87 772 
    Dr. Sevil Din?er  Yildiz Technical University, Faculty of Chemistry-Metallurgy, Istanbul, Turkey  sevild77@gmail.com  0090 21 24 49 17 78  0090 21 24 49 15 60 
    Prof. Dr. Claude P. Muller, Chair  The Institute of Immunology, Laboratoire National de Sant?, Luxembourg  claude.muller@LNS.ETAT.LU  00352 49 06 04  00352 49 06 86 


  • Description:
    WG2 Antigenicity.
    • Objective:
      Preparation of antibodies against relevant biological targets including microorganisms, biotoxins and proteins. This will allow full characterization of the microorganisms antigenic properties.
    • Description:
      This work package will bring together experts in the field of antigenicity, diagnostics and prophylaxis. Polyclonal antibodies, specific antibody fragments from phage display libraries, and mainly monoclonal antibodies (MAbs) will be developed/produced for a rapid, specific, sensitive and differentiating detection of bacteria, viruses and fungi, and bacterial and fungal toxins. Antibodies will be produced against bacteria, viruses and fungi, and derived products that fall within the scope of the project. In virology, special attention will be put on related microorganisms from the human and veterinary fields, with a special emphasis on poxviridae and BSL4 viruses. In addition, antibodies will be generated against peptides, proteins, glycoproteins, and protein-conjugated glycooligo- and polymers. This will allow the full antigenic properties of the microorganisms to be defined, which may also lead to a better understanding of the immunological reactions in infections.
      Antibodies will be produced against the biological targets using classical polyclonal antibody production in rabbits, classical hybridoma technology for the production of MAbs, and new technology for the production of specific antibody fragments by phage display libraries. The latter methodology is also called antibody engineering technology and is a new area in the field of molecular immunology for production of libraries of Fab fragments by bacterial expression of the genes coding for the VH and the VL domain and display of fragments on the surface of filamentous phage. Such phage display methodology permits a rapid construction of large combinatorial antibody libraries. The antibody genes can be recovered from lymphocytes mRNA by RT-PCR and then cloned.
  • Actions:

    Action
    Description
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  • Publications:

    Name
    Year
    Title
    Periodical
    Volume
    Page
    Pubmed
    MV La, P Barbry, D Raoult and P Renesto. 2007 Molecular basis of Tropheryma whipplei doxycycline susceptibility examined by transcriptional profiling. J Antimicrobial Chem in press / link to pubmed
    MF Ducatez, Y Guan, H Chen and CP Muller. 2007 Enhanced laboratory surveillance of group III coronaviruses in life poultry markets in Guangdong province, China, after the SARS outbreak. Proceedings of the 5th International Symposium on Avian Corona- and Pneumovirus, Rauischholzhausen, Germany. in press / link to pubmed
    P Renesto, C Abergel, P Decloquement, D Moinier, S Azza, H Ogata, P Fourquet, JP Gorvel and JM Claverie. 2006 Mimivirus giant particles incorporate a large fraction of anonymous and unique gene products. J Virol 80 11678-11685 link to pubmed
    N Crapoulet, P Barbry, D Raoult and P Renesto. 2006 Global transcriptome analysis of Tropheryma whipplei in response to temperature stresses. J Bacterio 188 5228-3529 link to pubmed
    H Ogata, B La Scola, S Audic, P Renesto, G Blanc, C Robert, PE Fournier, JM Claverie and D Raoult. 2006 Genome sequence of Rickettsia bellii illuminates the role of amebae in gene exchanges between intracellular pathogens. Plos Genet 2 0733-0744 link to pubmed
    P Renesto, L Samson, H Ogata, S Azza, P Fourquet, JP Gorvel, RA Heinzen and D Raoult. 2006 Identification of two putative rickettsial adhesins by proteomic analysis. Res Microbiol 157 605-612 link to pubmed
    M Sakarellos-Daitsiotis, D Krikorian, E Panou-Pomonis and C Sakarellos. 2006 Artificial Carriers: A Strategy for Constructing Antigenic/Immunogenic Conjugates. Curr Top in Med Chem 6 1715-1735 link to pubmed
    MF Ducatez, CM Olinger, AA Owoade, S De Landtsheer, W Ammerlaan, HG Niesters, AD Osterhaus, RA Fouchier and CP Muller. 2006 Avian flu: multiple introductions of H5N1 in Nigeria. Nature 442 37 link to pubmed
    CP Muller. 2006 H5N1. From the front lines: Nigeria. Nature, Nature News 440 726-727 link to pubmed
    AA Owoade, MF Ducatez and CP Muller. 2006 Seroprevalence of Avian Influenza Virus, Infectious Bronchitis Virus, Reovirus, Avian Pneumovirus, Infectious Laryngotracheitis Virus, and Avian Leukosis Virus in Nigerian Poultry. Avian Diseases 50 222-227 link to pubmed
    F Roman, W Ammerlaan, JM Pless?ria, SS Deroo, CP Muller, V Arendt, F Schneider, R Hemmer and JC Schmit. 2006 A new recombinant virus system for the study of HIV-1 entry and inhibition. J Virol Meth 131 99-104 link to pubmed
    MF Ducatez, AA Owoade, JO Abiola, CP Muller. 2006 Molecular Epidemiology of Chicken Anemia Virus in Nigeria. Arch Virol 151 97-111 link to pubmed
    P Crapoulet, S Robineau, D Raoult and P Renesto. 2005 Intervening Sequence Acquired by Lateral Gene Transfer in Tropheryma whipplei Results in 23S rRNA Fragmentation. Appl Environ Microbiol 71 6698-6701 link to pubmed
    H Ogata, P Renesto, S Audic , C Robert, G Blanc, PE Fournier, H Parinello, JM Claverie and D Raoult. 2005 The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite. PloS Biol 3 391-1402 link to pubmed
    P Renesto, S Azza, A Dolla, P Fourquet, G Vestris, JP Gorvel and D Raoult. 2005 Proteome analysis of Rickettsia conorii by two-dimensional gel electrophoresis coupled with mass spectrometry. FEMS Microbiol Lett 245 231-238 link to pubmed
    C Rovery, P Renesto, N Crapoulet , K Matsumoto, P Parola, H Ogata and D Raoult. 2005 Transcriptional response of Rickettsia conorii exposed to temperature variation and stress starvation. Res Microbiol 156 211-218 link to pubmed